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Anti-FRK Antibody

Western blot analysis of FRK expression in K562 (A), Molt4 (B), PC12 (C) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 54 kD)
Immunohistochemical analysis of FRK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FRK staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P42685
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	58254 Da

Additional Information
Gene ID	2444
Other Names	PTK5; RAK; Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FRK. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FRK
Synonyms	PTK5, RAK
Function	Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.
Cellular Location	Cytoplasm. Nucleus. Note=Predominantly found in the nucleus, with a small fraction found in the cell periphery
Tissue Location	Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Cance W.G.,et al.Cell Growth Differ. 5:1347-1355(1994).
Lee J.,et al.Gene 138:247-251(1994).
Mungall A.J.,et al.Nature 425:805-811(2003).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Cance W.G.,et al.Int. J. Cancer 54:571-577(1993).

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¥ 1,250.00

Cat# AP51213

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Anti-FRK Antibody

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