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Anti-Frizzled 5 Antibody

Western blot analysis of Frizzled 5 expression in A375 (A), mouse kidney (B), rat kidney (C), rat heart (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 65 kD)
Immunohistochemical analysis of Frizzled 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Frizzled 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q13467
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	64507 Da

Additional Information
Gene ID	7855
Other Names	C2orf31; Frizzled-5; Fz-5; hFz5; FzE5
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Frizzled 5. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FZD5
Synonyms	C2orf31
Function	Receptor for Wnt proteins (PubMed:10097073, PubMed:20530549, PubMed:26908622, PubMed:9054360). Functions in the canonical Wnt/beta- catenin signaling pathway. In vitro activates WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 signaling (By similarity). In neurons, activation by WNT7A promotes formation of synapses (PubMed:20530549). May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity). Plays a role in ocular development (PubMed:26908622).
Cellular Location	Cell membrane; Multi-pass membrane protein {ECO:0000250\|UniProtKB:Q8CHL0}. Golgi apparatus membrane {ECO:0000250\|UniProtKB:Q9EQD0}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:Q9EQD0}. Synapse {ECO:0000250\|UniProtKB:Q8CHL0}. Perikaryon {ECO:0000250\|UniProtKB:Q8CHL0}. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q8CHL0}. Cell projection, axon {ECO:0000250\|UniProtKB:Q8CHL0}. Note=Localized at the plasma membrane and also found at the Golgi apparatus. {ECO:0000250\|UniProtKB:Q9EQD0}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Wang Y.,et al.J. Biol. Chem. 271:4468-4476(1996).
Saitoh T.,et al.Int. J. Oncol. 19:105-110(2001).
Hillier L.W.,et al.Nature 434:724-731(2005).
Tanaka S.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:10164-10169(1998).
He X.,et al.Science 275:1652-1654(1997).

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Cat# AP51219

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Anti-Frizzled 5 Antibody

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