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Anti-GHRH Receptor Antibody

Western blot analysis of GHRH Receptor expression in mouse kidney (A), rat kidney (B) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47; 55 kD)
Immunofluorescent analysis of GHRH Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q02643
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	47402 Da

Additional Information
Gene ID	2692
Other Names	Growth hormone-releasing hormone receptor; GHRH receptor; Growth hormone-releasing factor receptor; GRF receptor; GRFR
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GHRH Receptor. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GHRHR
Function	Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	Pituitary gland.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Mayo K.E.,et al.Mol. Endocrinol. 6:1734-1744(1992).
Gaylinn B.D.,et al.Mol. Endocrinol. 7:77-84(1993).
Suwa M.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
King M.M.,et al.Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 424:157-164(2003).

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¥ 1,250.00

Cat# AP51229

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Anti-GHRH Receptor Antibody

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