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Anti-GPR110 Antibody

Western blot analysis of GPR110 expression in HEK293T (A), PC12 (B), CT26 (C), Hela (D) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 101 kD)
Immunofluorescent analysis of GPR110 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q5T601
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	101365 Da

Additional Information
Gene ID	266977
Other Names	PGR19; Probable G-protein coupled receptor 110; G-protein coupled receptor KPG_012; G-protein coupled receptor PGR19
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR110. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ADGRF1 {ECO:0000303\|PubMed:35418679, ECO:0000312\|HGNC:HGNC:18990}
Function	Adhesion G protein-coupled receptor (aGPCR) for N- docosahexaenoylethanolamine (synaptamide), an omega-3 fatty acid lipid highly enriched in the brain (PubMed:27759003, PubMed:32144388). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (PubMed:35418679, PubMed:36127364, PubMed:37120430). ADGRF1 is coupled to G(s) G proteins and mediates activation of adenylate cyclase activity (PubMed:35418679). Also able to couple to G(q), G(i) and G(12)/G(13) G proteins; additional evidence is however required to confirm this result in vivo (PubMed:36127364, PubMed:37120430). Involved in the development of neurons and cognitive function (By similarity). In liver, involved in fat accumulation (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Mainly expressed in the kidney. Up-regulated in lung adenocarcinomas and prostate cancers

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Suwa M.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Okazaki H.,et al.Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mungall A.J.,et al.Nature 425:805-811(2003).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP51235

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Anti-GPR110 Antibody

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