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Anti-GPR153 Antibody

Western blot analysis of GPR153 expression in A549 (A), HeLa (B), Raw264.7 (C), rat lung (D) whole cell lysates. (Predicted band size: 65 kD; Observed band size: 65 kD)
Immunofluorescent analysis of GPR153 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q6NV75
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	65361 Da

Additional Information
Gene ID	387509
Other Names	PGR1; Probable G-protein coupled receptor 153; G-protein coupled receptor PGR1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR153. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GPR153
Synonyms	PGR1
Function	Orphan receptor.
Cellular Location	Cell membrane; Multi-pass membrane protein.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Gregory S.G.,et al.Nature 441:315-321(2006).
Vassilatis D.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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¥ 1,250.00

Cat# AP51236

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Anti-GPR153 Antibody

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