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Anti-mGLUR7 Antibody

Western blot analysis of mGLUR7 expression in U2OS (A), mouse testis (B), rat testis (C) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 102 kD)
Immunohistochemical analysis of mGLUR7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of mGLUR7 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP, IF/IC
Primary Accession	Q14831
Reactivity	Human, Mouse, Rat, Rabbit, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	102251 Da

Additional Information
Gene ID	2917
Other Names	GPRC1G; MGLUR7; Metabotropic glutamate receptor 7; mGluR7
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human mGLUR7. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IP~~N/A IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GRM7
Synonyms	GPRC1G, MGLUR7
Function	G protein-coupled receptor activated by glutamate that regulates axon outgrowth through the MAPK-cAMP-PKA signaling pathway during neuronal development (PubMed:33500274). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide- binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase that it inhibits (PubMed:9473604).
Cellular Location	Cell membrane; Multi-pass membrane protein
Tissue Location	Expressed in many areas of the brain, especially in the cerebral cortex, hippocampus, and cerebellum. Expression of GRM7 isoforms in non-neuronal tissues appears to be restricted to isoform 3 and isoform 4.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Makoff A.,et al.Brain Res. Mol. Brain Res. 40:165-170(1996).
Flor P.J.,et al.Neuropharmacology 36:153-159(1997).
Wu S.,et al.Brain Res. Mol. Brain Res. 53:88-97(1998).
Schulz H.L.,et al.Neurosci. Lett. 326:37-40(2002).
Bolonna A.A.,et al.Schizophr. Res. 47:99-103(2001).

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¥ 1,250.00

Cat# AP51248

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100 µl

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Anti-mGLUR7 Antibody

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