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Anti-GUCY1A3 Antibody

Western blot analysis of GUCY1A3 expression in HEK293T (A), Hela (B), H446 (C), mouse lung (D), mouse kidney (E), rat kidney (F) whole cell lysates. (Predicted band size: 77 kD; Observed band size: 77; 70 kD)
Immunohistochemical analysis of GUCY1A3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q02108
Reactivity	Human, Mouse, Rat, Pig, Bovine, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	77452 Da

Additional Information
Gene ID	2982
Other Names	GUC1A3; GUCSA3; GUCY1A1; Guanylate cyclase soluble subunit alpha-3; GCS-alpha-3; GCS-alpha-1; Soluble guanylate cyclase large subunit
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GUCY1A3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GUCY1A1 (HGNC:4685)
Cellular Location	Cytoplasm.
Tissue Location	Detected in brain cortex and lung (at protein level).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Giuili G.,et al.FEBS Lett. 304:83-88(1992).
Gansemans Y.,et al.Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
Zabel U.,et al.Biochem. J. 335:51-57(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).

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¥ 1,250.00

Cat# AP51251

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100 µl

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¥ 2,050.00

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Anti-GUCY1A3 Antibody

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