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Anti-HOXB5 Antibody

Western blot analysis of HOXB5 expression in PC12 (A), Hela (B), HCT116 (C) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 37 kD)
Immunofluorescent analysis of HOXB5 staining in H460 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P09067
Reactivity	Human, Mouse, Rat, Zebrafish, Chicken
Host	Rabbit
Clonality	Polyclonal
Calculated MW	29434 Da

Additional Information
Gene ID	3215
Other Names	HOX2A; Homeobox protein Hox-B5; Homeobox protein HHO.C10; Homeobox protein Hox-2A; Homeobox protein Hu-1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HOXB5. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HOXB5
Synonyms	HOX2A
Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location	Nucleus.
Tissue Location	Spinal cord.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Galang C.K.,et al.New Biol. 4:558-568(1992).
Kidd K.K.,et al.Am. J. Hum. Genet. 67:235-235(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hauser C.A.,et al.Cell 43:19-28(1985).
Levine M.,et al.Cell 38:667-673(1984).

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¥ 1,250.00

Cat# AP51265

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Anti-HOXB5 Antibody

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