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Anti-HOXD12 Antibody

Western blot analysis of HOXD12 expression in HEK293T (A), SHSY5Y (B), mouse liver (C), rat liver (D) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 22 kD)
Immunohistochemical analysis of HOXD12 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P35452
Reactivity	Human, Mouse, Rat, Pig, Chicken, Bovine, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	29031 Da

Additional Information
Gene ID	3238
Other Names	HOX4H; Homeobox protein Hox-D12; Homeobox protein Hox-4H
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HOXD12. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HOXD12
Synonyms	HOX4H
Function	Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location	Nucleus.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Miwa N.,et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
D'Esposito M.,et al.Genomics 10:43-50(1991).

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¥ 1,250.00

Cat# AP51267

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Anti-HOXD12 Antibody

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