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Anti-Involucrin Antibody

Western blot analysis of Involucrin expression in HEK293T (A), H1792 (B) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 68 kD)
Immunohistochemical analysis of Involucrin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Involucrin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P07476
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	68479 Da

Additional Information
Gene ID	3713
Other Names	Involucrin
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Involucrin. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	IVL
Function	Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Cellular Location	Cytoplasm. Note=Constituent of the scaffolding of the cornified envelope
Tissue Location	Keratinocytes of epidermis and other stratified squamous epithelia

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Eckert R.L.,et al.Cell 46:583-589(1986).
Gregory S.G.,et al.Nature 441:315-321(2006).
Robinson N.A.,et al.J. Biol. Chem. 272:12035-12046(1997).
Yaffe M.B.,et al.J. Biol. Chem. 267:12233-12238(1992).
Simon M.,et al.J. Biol. Chem. 263:18093-18098(1988).

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¥ 1,250.00

Cat# AP51294

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Anti-Involucrin Antibody

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