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Anti-Kv3.4 Antibody

Western blot analysis of Kv3.4 expression in rat brain (A) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 70 kD)
Immunohistochemical analysis of Kv3.4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Kv3.4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q03721
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	69767 Da

Additional Information
Gene ID	3749
Other Names	Potassium voltage-gated channel subfamily C member 4; KSHIIIC; Voltage-gated potassium channel subunit Kv3.4
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Kv3.4. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KCNC4 (HGNC:6236)
Function	Voltage-gated potassium channel that opens in response to the voltage difference across the membrane, forming a potassium-selective channel through which potassium ions pass in accordance with their electrochemical gradient (PubMed:7993631). The channel displays rapid activation and inactivation kinetics (PubMed:7993631).
Cellular Location	Membrane; Multi-pass membrane protein.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

de Miera E.V.-S.,et al.Proc. R. Soc. B 248:9-18(1992).
Gregory S.G.,et al.Nature 441:315-321(2006).
Covarrubias M.,et al.Neuron 13:1403-1412(1994).
Beck E.J.,et al.J. Gen. Physiol. 112:71-84(1998).
Antz C.,et al.Nature 385:272-275(1997).

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¥ 1,250.00

Cat# AP51299

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Anti-Kv3.4 Antibody

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