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Anti-Cytokeratin 14 Antibody

Western blot analysis of Cytokeratin 14 expression in MCF7 (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 52 kD)
Immunohistochemical analysis of Cytokeratin 14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytokeratin 14 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P02533
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	51561 Da

Additional Information
Gene ID	3861
Other Names	KRT14; Keratin, type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Cytokeratin 14. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KRT14 (HGNC:6416)
Function	Structural component of intermediate filaments in basal keratinocytes of stratified epithelia. Forms obligate heteropolymers with the type II keratin KRT5, contributing to the keratin intermediate filament network that confers mechanical strength and structural integrity to the basal layer of the epidermis and other stratified epithelial (PubMed:11724817, PubMed:1694855, PubMed:1720261). The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro (PubMed:11724817).
Cellular Location	Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern.
Tissue Location	Expressed in the corneal epithelium (at protein level) (PubMed:26758872). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair (PubMed:9457912). Found in keratinocytes surrounding the club hair during telogen (PubMed:9457912).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Marchuk D.,et al.Cell 39:491-498(1984).
Marchuk D.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:1609-1613(1985).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Zody M.C.,et al.Nature 440:1045-1049(2006).
Hanukoglu I.,et al.Cell 31:243-252(1982).

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Cat# AP51307

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Anti-Cytokeratin 14 Antibody

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