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Anti-Cytokeratin 16 Antibody

Western blot analysis of Cytokeratin 16 expression in H446 (A), mouse lung (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)
Immunohistochemical analysis of Cytokeratin 16 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytokeratin 16 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P08779
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	51268 Da

Additional Information
Gene ID	3868
Other Names	KRT16A; Keratin, type I cytoskeletal 16; Cytokeratin-16; CK-16; Keratin-16; K16
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 16. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KRT16
Synonyms	KRT16A
Function	Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.
Tissue Location	Expressed in the corneal epithelium (at protein level).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Raychaudhury A.,et al.Mol. Cell. Biol. 6:539-548(1986).
Rosenberg M.,et al.Mol. Cell. Biol. 8:722-736(1988).
Paladini R.D.,et al.Biochem. Biophys. Res. Commun. 215:517-523(1995).
Smith F.J.D.,et al.Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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Cat# AP51308

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Anti-Cytokeratin 16 Antibody

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