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Anti-EDG7 Antibody

Western blot analysis of EDG7 expression in HepG2 (A), NT2D1 (B) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 40 kD)
Immunohistochemical analysis of EDG7 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9UBY5
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	40128 Da

Additional Information
Gene ID	23566
Other Names	EDG7; LPA3; Lysophosphatidic acid receptor 3; LPA receptor 3; LPA-3; Lysophosphatidic acid receptor Edg-7
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EDG7. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	LPAR3
Synonyms	EDG7, LPA3
Function	Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. May play a role in the development of ovarian cancer. Seems to be coupled to the G(i)/G(o) and G(q) families of heteromeric G proteins.
Cellular Location	Cell membrane; Multi-pass membrane protein.
Tissue Location	Most abundantly expressed in prostate, testes, pancreas, and heart, with moderate levels in lung and ovary. No detectable expression in brain, placenta, liver, skeletal muscle, kidney, spleen, thymus, small intestine, colon, or peripheral blood leukocytes

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Bandoh K.,et al.J. Biol. Chem. 274:27776-27785(1999).
Im D.-S.,et al.Mol. Pharmacol. 57:753-759(2000).
Kopatz S.A.,et al.Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
Contos J.J.A.,et al.Mol. Pharmacol. 58:1188-1196(2000).

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¥ 1,250.00

Cat# AP51321

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Anti-EDG7 Antibody

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