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Anti-MASP1 HC Antibody

Western blot analysis of MASP1 HC expression in LO2 (A), H9C2 (B), K562 (C) whole cell lysates. (Predicted band size: 79 kD; Observed band size: 49 kD)
Immunofluorescent analysis of MASP1 HC staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P48740
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	79247 Da

Additional Information
Gene ID	5648
Other Names	CRARF; CRARF1; PRSS5; Mannan-binding lectin serine protease 1; Complement factor MASP-3; Complement-activating component of Ra-reactive factor; Mannose-binding lectin-associated serine protease 1; MASP-1; Mannose-binding protein-associated serine protease; Ra-reactive factor serine protease p100; RaRF; Serine protease 5
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MASP1 HC. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MASP1 {ECO:0000303\|PubMed:11290788, ECO:0000312\|HGNC:HGNC:6901}
Function	Precursor of a serum protease that activates the complement pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.
Cellular Location	Secreted. [Isoform MASP-3]: Secreted
Tissue Location	Protein of the plasma which is primarily expressed by liver.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Takada F.,et al.Biochem. Biophys. Res. Commun. 196:1003-1009(1993).
Sato T.,et al.Int. Immunol. 6:665-669(1994).
Endo Y.,et al.Int. Immunol. 8:1355-1358(1996).
Takayama Y.,et al.Mol. Immunol. 36:505-514(1999).
Dahl M.R.,et al.Immunity 15:127-135(2001).

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¥ 1,250.00

Cat# AP51330

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Anti-MASP1 HC Antibody

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