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Anti-GANP Antibody

Western blot analysis of GANP expression in rat testis (A), CT26 (B), SKOVCAR3 (C), HCT116 (D), HEK293T (E), HCC827 (F) whole cell lysates. (Predicted band size: 80; 218 kD; Observed band size: 80; 218 kD)
Immunohistochemical analysis of GANP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	O60318
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	218405 Da

Additional Information
Gene ID	8888
Other Names	GANP; KIAA0572; MAP80; Germinal-center associated nuclear protein; GANP; 80 kDa MCM3-associated protein; MCM3 acetylating protein; MCM3AP; MCM3 acetyltransferase
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GANP. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MCM3AP
Synonyms	GANP, KIAA0572, MAP80
Function	[Isoform GANP]: As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores (PubMed:20005110, PubMed:20384790, PubMed:22307388, PubMed:23591820). Through the acetylation of histones, affects the assembly of nucleosomes at immunoglobulin variable region genes and promotes the recruitment and positioning of transcription complex to favor DNA cytosine deaminase AICDA/AID targeting, hence promoting somatic hypermutations (PubMed:23652018).
Cellular Location	[Isoform GANP]: Nucleus envelope. Nucleus, nuclear pore complex. Nucleus, nucleoplasm. Chromosome. Note=Predominantly located at the nuclear envelope, facing the nucleus interior (PubMed:20005110, PubMed:21195085, PubMed:23591820). Localization at the nuclear pore complex requires NUP153, TPR and ALYREF/ALY (PubMed:22307388, PubMed:23591820). Also found associated with chromatin (PubMed:23652018). In B-cells, targeted to the immunoglobulin variable region genes (PubMed:23652018).
Tissue Location	Widely expressed (PubMed:11024281). Up-regulated in germinal center B-cells in tonsils (at protein level) (PubMed:11024281).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Abe E.,et al.Gene 255:219-227(2000).
Hattori M.,et al.Nature 405:311-319(2000).
Nagase T.,et al.DNA Res. 5:31-39(1998).
Takei Y.,et al.J. Biol. Chem. 273:22177-22180(1998).
Takei Y.,et al.EMBO Rep. 2:119-123(2001).

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Cat# AP51336

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Anti-GANP Antibody

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