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Anti-MMP15 Antibody

Western blot analysis of MMP15 expression in HEK293T (A), Hela (B), H446 (C) whole cell lysates. (Predicted band size: 75 kD; Observed band size: 75 kD)
Immunofluorescent analysis of MMP15 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P51511
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	75807 Da

Additional Information
Gene ID	4324
Other Names	Matrix metalloproteinase-15; MMP-15; Membrane-type matrix metalloproteinase 2; MT-MMP 2; MTMMP2; Membrane-type-2 matrix metalloproteinase; MT2-MMP; MT2MMP; SMCP-2
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MMP15. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MMP15
Function	Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
Cellular Location	Membrane; Single-pass type I membrane protein; Extracellular side
Tissue Location	Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Will H.,et al.Eur. J. Biochem. 231:602-608(1995).
Sato H.,et al.Genomics 39:412-413(1997).
Shofuda K.,et al.Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
d'Ortho M.P.,et al.Eur. J. Biochem. 250:751-757(1997).
Daub H.,et al.Mol. Cell 31:438-448(2008).

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Cat# AP51351

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Anti-MMP15 Antibody

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