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Anti-NEFL Antibody

Western blot analysis of NEFL expression in Hela (A), HGC27 (B), mouse brain (C) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 70 kD)
Immunofluorescent analysis of NEFL staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P07196
Reactivity	Human, Mouse, Rat, Pig, Bovine, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	61517 Da

Additional Information
Gene ID	4747
Other Names	NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NEFL. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NEFL
Synonyms	NF68, NFL
Function	Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Cellular Location	Cell projection, axon {ECO:0000250\|UniProtKB:P08551}. Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:P08551}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Julien J.-P.,et al.Biochim. Biophys. Acta 909:10-20(1987).
Perez-Olle R.,et al.J. Cell Sci. 115:4937-4946(2002).
Nusbaum C.,et al.Nature 439:331-335(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Pospelov V.A.,et al.Cell Growth Differ. 5:187-196(1994).

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¥ 1,250.00

Cat# AP51382

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Anti-NEFL Antibody

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