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Anti-PMS1 Antibody

Western blot analysis of PMS1 expression in mouse testis (A), rat lung (B) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 106 kD)
Immunohistochemical analysis of PMS1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P54277
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	105830 Da

Additional Information
Gene ID	5378
Other Names	PMSL1; PMS1 protein homolog 1; DNA mismatch repair protein PMS1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PMS1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	PMS1
Synonyms	PMSL1
Function	Probably involved in the repair of mismatches in DNA.
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00267}.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Nicolaides N.C.,et al.Nature 371:75-80(1994).
Hayashi A.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Leung W.K.,et al.J. Biol. Chem. 275:15728-15732(2000).

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¥ 1,250.00

Cat# AP51433

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Anti-PMS1 Antibody

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