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Anti-Surfactant Protein B Antibody

Western blot analysis of Surfactant Protein B expression in A549 (A) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
Immunofluorescent analysis of Surfactant Protein B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P07988
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42117 Da

Additional Information
Gene ID	6439
Other Names	SFTP3; Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe)
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Surfactant Protein B. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SFTPB
Synonyms	SFTP3
Function	Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
Cellular Location	Secreted, extracellular space, surface film.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Jacobs K.A.,et al.J. Biol. Chem. 262:9808-9811(1987).
Pilot-Matias T.J.,et al.DNA 8:75-86(1989).
Glasser S.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:4007-4011(1987).
Revak S.D.,et al.J. Clin. Invest. 81:826-833(1988).
Johansson J.,et al.FEBS Lett. 301:165-167(1992).

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¥ 1,250.00

Cat# AP51509

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Anti-Surfactant Protein B Antibody

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