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Anti-SRSF4 Antibody

Western blot analysis of SRSF4 expression in H1792 (A), Beas2B (B), SGC7901 (C), EC9706 (D), HEK293T (E), mouse kidney (F), rat heart (G) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)
Immunohistochemical analysis of SRSF4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q08170
Reactivity	Human, Mouse, Rat, Pig, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	56678 Da

Additional Information
Gene ID	6429
Other Names	SFRS4; SRP75; Serine/arginine-rich splicing factor 4; Pre-mRNA-splicing factor SRP75; SRP001LB; Splicing factor, arginine/serine-rich 4
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SRSF4. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SRSF4
Synonyms	SFRS4, SRP75
Function	Plays a role in alternative splice site selection during pre- mRNA splicing. Represses the splicing of MAPT/Tau exon 10.
Cellular Location	Nucleus speckle.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Zahler A.M.,et al.Mol. Cell. Biol. 13:4023-4028(1993).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
Connolly K.S.,et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Zahler A.M.,et al.Genes Dev. 6:837-847(1992).

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¥ 1,250.00

Cat# AP51534

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100 µl

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Anti-SRSF4 Antibody

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