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Anti-Striatin Antibody

Western blot analysis of Striatin expression in BV2 (A), PC12 (B), HEK293T (C), U87MG (D) whole cell lysates. (Predicted band size: 86 kD; Observed band size: 86 kD)
Immunofluorescent analysis of Striatin staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	O43815
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	86132 Da

Additional Information
Gene ID	6801
Other Names	Striatin
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Striatin. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	STRN
Function	Calmodulin-binding scaffolding protein which is the center of the striatin-interacting phosphatase and kinase (STRIPAK) complexes (PubMed:18782753). STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (Probable).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:P70483}. Membrane {ECO:0000250\|UniProtKB:P70483}; Peripheral membrane protein {ECO:0000250\|UniProtKB:P70483}. Cell projection, dendritic spine {ECO:0000250\|UniProtKB:P70483}. Note=CTTNBP2-binding may regulate dendritic spine distribution. {ECO:0000250\|UniProtKB:P70483}
Tissue Location	Preferentially expressed in brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Moqrich A.,et al.Genomics 51:136-139(1998).
Hillier L.W.,et al.Nature 434:724-731(2005).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).
Oppermann F.S.,et al.Mol. Cell. Proteomics 8:1751-1764(2009).

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Cat# AP51542

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Anti-Striatin Antibody

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