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Anti-CD256 Antibody

Western blot analysis of CD256 expression in HEK293T (A), Hela (B), H446 (C), mouse brain (D), mouse kidney (E), rat brain (F), rat kidney (G) whole cell lysates. (Predicted band size: 22; 24; 25; 27; 36 kD; Observed band size: 22 kD)
Immunohistochemical analysis of CD256 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CD256 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	O75888
Reactivity	Human, Mouse, Rat, Pig, Bovine, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	27433 Da

Additional Information
Gene ID	8741
Other Names	APRIL; TALL2; ZTNF2; Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD256
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD256. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TNFSF13
Synonyms	APRIL, TALL2, ZTNF2
Function	Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
Cellular Location	Secreted.
Tissue Location	Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Hahne M.,et al.J. Exp. Med. 188:1185-1190(1998).
Shu H.-B.,et al.J. Leukoc. Biol. 65:680-683(1999).
Farrah T.,et al.Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Kelly K.A.,et al.Cancer Res. 60:1021-1027(2000).
Pradet-Balade B.,et al.EMBO J. 21:5711-5720(2002).

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¥ 1,250.00

Cat# AP51575

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Anti-CD256 Antibody

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