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Anti-Tensin 1 (Phospho-Y1326) Antibody

Western blot analysis of Tensin 1 (Phospho-Y1326) expression in MEF (A), PC12 (B), LOVO (C) whole cell lysates. (Predicted band size: 185 kD; Observed band size: 200 kD)
Immunofluorescent analysis of Tensin 1 (Phospho-Y1326) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9HBL0
Reactivity	Human, Mouse, Rat, Pig
Host	Rabbit
Clonality	Polyclonal
Calculated MW	197023 Da

Additional Information
Gene ID	7145
Other Names	TNS; Tensin-1
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1326 of human Tensin 1 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TNS1
Synonyms	TNS
Function	May act as a protein phosphatase and/or a lipid phosphatase (Probable). Involved in fibrillar adhesion formation (PubMed:21768292, PubMed:28005397). Essential for myofibroblast differentiation and myofibroblast-mediated extracellular matrix deposition (PubMed:28005397). Enhances RHOA activation in the presence of DLC1 (PubMed:26427649). Plays a role in cell polarization and migration (PubMed:19826001). May be involved in cartilage development and in linking signal transduction pathways to the cytoskeleton (PubMed:21768292).
Cellular Location	Cell surface. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=Localizes to both focal adhesions and fibrillar adhesions (PubMed:20069572). Localized at the cell periphery preferentially to fibrillar adhesions rather than to focal adhesions. Translocates from the cell edge to the cell center in an ITGB1BP1-dependent manner (PubMed:21768292)
Tissue Location	In the lung, detected in the alveolar septa (at protein level) (PubMed:28005397). Ubiquitous (PubMed:11023826)

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Chen H.,et al.Biochem. J. 351:403-411(2000).
Hillier L.W.,et al.Nature 434:724-731(2005).
Bienvenut W.V.,et al.Submitted (OCT-2009) to UniProtKB.
Qian X.,et al.Proc. Natl. Acad. Sci. U.S.A. 104:9012-9017(2007).
Cantin G.T.,et al.J. Proteome Res. 7:1346-1351(2008).

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¥ 1,250.00

Cat# AP51576

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Anti-Tensin 1 (Phospho-Y1326) Antibody

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