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Anti-Tyrosinase Antibody

Western blot analysis of Tyrosinase expression in A375 (A), Podocyte (B), Beas2B (C), PMVEC (D), BV2 (E) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 70 kD)
Immunohistochemical analysis of Tyrosinase staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Tyrosinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P14679
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	60393 Da

Additional Information
Gene ID	7299
Other Names	Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Tyrosinase. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TYR (HGNC:12442)
Function	This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine (By similarity). In addition to hydroxylating tyrosine to DOPA (3,4- dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA- quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone (PubMed:28661582).
Cellular Location	Melanosome membrane; Single-pass type I membrane protein. Melanosome {ECO:0000250\|UniProtKB:P11344}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38 {ECO:0000250\|UniProtKB:P11344}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Giebel L.B.,et al.Genomics 9:435-445(1991).
Kwon B.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:7473-7477(1987).
Kwon B.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:6352-6352(1988).
Bouchard B.,et al.J. Exp. Med. 169:2029-2042(1989).
Chintamaneni C.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:5272-5276(1991).

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Cat# AP51594

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Anti-Tyrosinase Antibody

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