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Anti-14-3-3 gamma Antibody [KO/KD Validated]

Western blot analysis of 14-3-3 gamma expression in HEK293T (A), A549 (B), MCF7 (C), mouse lung (D), mouse liver (E), rat lung (F), rat liver (G) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 28 kD)
Immunohistochemical analysis of 14-3-3 gamma staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of 14-3-3 gamma staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of 14-3-3 gamma expression in wild type (WT) and knockdown (KD) HeLa cell lysates.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P61981
Reactivity	Human, Mouse, Rat, Zebrafish, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	28303 Da

Additional Information
Gene ID	7532
Other Names	14-3-3 protein gamma; Protein kinase C inhibitor protein 1; KCIP-1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 14-3-3 gamma. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	YWHAG (HGNC:12852)
Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed:16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed:36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L- glutamate secretion through interaction with BEST1 (PubMed:29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed:29678907). Plays a role in mitochondrial protein catabolic process (also named MALM) that promotes the degradation of damaged proteins inside mitochondria (PubMed:22532927).
Cellular Location	Cytoplasm, cytosol. Mitochondrion matrix. Note=Translocates to the mitochondrial matrix following induction of MALM (mitochondrial protein catabolic process).
Tissue Location	Highly expressed in brain, skeletal muscle, and heart.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Autieri M.V.,et al.DNA Cell Biol. 18:555-564(1999).
Horie M.,et al.Genomics 60:241-243(1999).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 424:157-164(2003).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.

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Cat# AP51619

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Anti-14-3-3 gamma Antibody [KO/KD Validated]

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