> 首页 > 产品 > 一抗 > 神经科学 > Anti-GLUR4 (Phospho-S862) Antibody

Anti-GLUR4 (Phospho-S862) Antibody

Western blot analysis of GLUR4 (Phospho-S862) expression in HEK293T (A), rat brain (B), mouse brain (C), MCF7 (D) whole cell lysates. (Predicted band size: 100 kD; Observed band size: 100 kD)
Immunohistochemical analysis of GLUR4 (Phospho-S862) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of GLUR4 (Phospho-S862) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P48058
Reactivity	Human, Mouse, Rat, Zebrafish, Chicken, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	100871 Da

Additional Information
Gene ID	2893
Other Names	GLUR4; Glutamate receptor 4; GluR-4; GluR4; AMPA-selective glutamate receptor 4; GluR-D; Glutamate receptor ionotropic, AMPA 4; GluA4
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S862 of human GLUR4 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GRIA4 {ECO:0000303\|PubMed:29220673, ECO:0000312\|HGNC:HGNC:4574}
Function	Ionotropic glutamate receptor that functions as a ligand- gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (By similarity). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (By similarity). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (PubMed:21172611).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P19493}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P19493} Postsynaptic cell membrane {ECO:0000250\|UniProtKB:P19493}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P19493}. Cell projection, dendrite {ECO:0000250\|UniProtKB:P19493}. Postsynaptic cell membrane {ECO:0000250\|UniProtKB:P42262}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P42262}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol