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Anti-Phospholipase D2 (Phospho-Y169) Antibody

     
  • 1 - Anti-Phospholipase D2 (Phospho-Y169) Antibody AP51658
    Western blot analysis of Phospholipase D2 (Phospho-Y169) expression in HEK293T TNFa-treated (A), NIHC3T3 TNFa-treated (B), H9C2 TNFa-treated (C) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 105 kD)
  • 19 - Anti-Phospholipase D2 (Phospho-Y169) Antibody AP51658
    Immunohistochemical analysis of Phospholipase D2 (Phospho-Y169) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession O14939
Reactivity Human, Mouse, Rat, Bovine, Mk
Host Rabbit
Clonality Polyclonal
Calculated MW 105987 Da
Additional Information
Gene ID 5338
Other Names Phospholipase D2; PLD 2; hPLD2; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2
Target/Specificity KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y169 of human Phospholipase D2 protein. The exact sequence is proprietary.
Dilution WB~~WB (1/500 - 1/1000)
IHC~~1:100~500
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name PLD2 (HGNC:9068)
Function Function as phospholipase selective for phosphatidylcholine (PubMed:9582313). May have a role in signal-induced cytoskeletal regulation and/or endocytosis (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P97813}; Lipid-anchor {ECO:0000250|UniProtKB:P97813}
Tissue Location Ubiquitous..
Research Areas

REFERENCES

Steed P.M.,et al.FASEB J. 12:1309-1317(1998).
Lopez I.,et al.J. Biol. Chem. 273:12846-12852(1998).
Saqib K.M.,et al.Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
Divecha N.,et al.EMBO J. 19:5440-5449(2000).
Sjoeblom T.,et al.Science 314:268-274(2006).

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