Anti-ADAMTS2 Antibody

Western blot analysis of ADAMTS2 expression in C6 (A), CT26 (B), Hela (C) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 110 kD)
Immunohistochemical analysis of ADAMTS2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	O95450
Reactivity	Human, Mouse, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	134755 Da

Additional Information
Gene ID	9509
Other Names	PCINP; PCPNI; A disintegrin and metalloproteinase with thrombospondin motifs 2; ADAM-TS 2; ADAM-TS2; ADAMTS-2; Procollagen I N-proteinase; PC I-NP; Procollagen I/II amino propeptide-processing enzyme; Procollagen N-endopeptidase; pNPI
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ADAMTS2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ADAMTS2
Synonyms	PCINP, PCPNI
Function	Cleaves the propeptides of type I and II collagen prior to fibril assembly (By similarity). Does not act on type III collagen (By similarity). Cleaves lysyl oxidase LOX at a site downstream of its propeptide cleavage site to produce a short LOX form with reduced collagen-binding activity (PubMed:31152061).
Cellular Location	Secreted, extracellular space, extracellular matrix
Tissue Location	Expressed at high level in skin, bone, tendon and aorta and at low levels in thymus and brain

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.