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Anti-ALDH3B2 Antibody

Western blot analysis of ALDH3B2 expression in mouse brain (A), SGC7901 (B), A549 (C), U87MG (D) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 48 kD)
Immunofluorescent analysis of ALDH3B2 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P48448
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42635 Da

Additional Information
Gene ID	222
Other Names	ALDH8; Aldehyde dehydrogenase family 3 member B2; Aldehyde dehydrogenase 8
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ALDH3B2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ALDH3B2
Synonyms	ALDH8
Function	Oxidizes medium and long chain fatty aldehydes in lipid droplets into non-toxic fatty acids.
Cellular Location	Lipid droplet {ECO:0000250\|UniProtKB:E9Q3E1}.
Tissue Location	Salivary gland (PubMed:8890755). Expressed at protein level in placenta (PubMed:31847104)

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Hsu L.C.,et al.Adv. Exp. Med. Biol. 372:159-168(1995).
Hsu L.C.,et al.Gene 174:319-322(1996).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

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¥ 1,250.00

Cat# AP51720

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Anti-ALDH3B2 Antibody

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