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Anti-CD158b1 Antibody

Western blot analysis of CD158b1 expression in MDAMB231 (A), Jurkat (B), mouse liver (C), H9C2 (D) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 58 kD)
Immunofluorescent analysis of CD158b1 staining in RAW264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P43627
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	38472 Da

Additional Information
Gene ID	3803
Other Names	CD158B1; NKAT6; Killer cell immunoglobulin-like receptor 2DL2; CD158 antigen-like family member B1; MHC class I NK cell receptor; Natural killer-associated transcript 6; NKAT-6; p58 natural killer cell receptor clone CL-43; p58 NK receptor CL-43; CD158b1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD158b1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	KIR2DL2 (HGNC:6330)
Synonyms	CD158B1, NKAT6
Function	Receptor on natural killer (NK) cells for HLA-Cw1, 3, 7, and 8 allotypes. Inhibits the activity of NK cells thus preventing cell lysis.
Cellular Location	Cell membrane; Single-pass type I membrane protein

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Wagtmann N.,et al.Immunity 2:439-449(1995).
Doehring C.,et al.Immunogenetics 44:227-230(1996).
Hou L.,et al.Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
Snyder G.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:3864-3869(1999).
Boyington J.C.,et al.Nature 405:537-543(2000).

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Cat# AP51783

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Anti-CD158b1 Antibody

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