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Anti-NDUFA9 Antibody

Western blot analysis of NDUFA9 expression in Hela (A), A375 (B), mouse heart (C), mouse brain (D), rat heart (E), rat brain (F) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 37 kD)
Immunohistochemical analysis of NDUFA9 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of NDUFA9 staining in RAW264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q16795
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42510 Da

Additional Information
Gene ID	4704
Other Names	NDUFS2L; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 mitochondrial; Complex I-39kD; CI-39kD; NADH-ubiquinone oxidoreductase 39 kDa subunit
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NDUFA9. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NDUFA9
Synonyms	NDUFS2L
Function	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Required for proper complex I assembly (PubMed:28671271). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Location	Mitochondrion matrix

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Loeffen J.L.C.M.,et al.Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
Cross S.H.,et al.Nat. Genet. 6:236-244(1994).
Baens M.,et al.Genomics 16:214-218(1993).
Murray J.,et al.J. Biol. Chem. 278:13619-13622(2003).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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Cat# AP51800

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Anti-NDUFA9 Antibody

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