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Anti-CD42c Antibody

Western blot analysis of CD42c expression in mouse testis (A), mouse heart (B), rat testis (C), rat heart (D) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 22 kD)
Immunofluorescent analysis of CD42c staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P13224
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	21718 Da

Additional Information
Gene ID	2812
Other Names	Platelet glycoprotein Ib beta chain; GP-Ib beta; GPIb-beta; GPIbB; Antigen CD42b-beta; CD42c
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD42c. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GP1BB
Function	Gp-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to von Willebrand factor, which is already bound to the subendothelium.
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Expressed in heart and brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Lopez J.A.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:2135-2139(1988).
Yagi M.,et al.J. Biol. Chem. 269:17424-17427(1994).
Kelly M.D.,et al.J. Clin. Invest. 93:2417-2424(1994).
Zieger B.,et al.J. Clin. Invest. 99:520-525(1997).
Kunishima S.,et al.Blood 89:2404-2412(1997).

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Cat# AP51819

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Anti-CD42c Antibody

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