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Anti-SLC6A8 Antibody

Western blot analysis of SLC6A8 expression in MDAMB435 (A), Raw264.7 (B), H9C2 (C) whole cell lysates. (Predicted band size: 30; 58; 70; 80 kD; Observed band size: 70 kD)
Immunofluorescent analysis of SLC6A8 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P48029
Reactivity	Human, Mouse, Rat, Rabbit
Host	Rabbit
Clonality	Polyclonal
Calculated MW	70523 Da

Additional Information
Gene ID	6535
Other Names	Sodium- and chloride-dependent creatine transporter 1; CT1; Creatine transporter 1; Solute carrier family 6 member 8
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SLC6A8. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SLC6A8
Function	Creatine:sodium symporter which mediates the uptake of creatine (PubMed:17465020, PubMed:22644605, PubMed:25861866, PubMed:7945388, PubMed:7953292, PubMed:9882430). Plays an important role in supplying creatine to the brain via the blood-brain barrier (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein
Tissue Location	Predominantly expressed in skeletal muscle and kidney. Also found in brain, heart, colon, testis and prostate

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Nash S.R.,et al.Recept. Channels 2:165-174(1994).
Sora I.,et al.Biochem. Biophys. Res. Commun. 204:419-427(1994).
Sandoval N.,et al.Genomics 35:383-385(1996).
Barnwell L.F.,et al.Gene 159:287-288(1995).
Martinez-Munoz C.,et al.Gene 418:53-59(2008).

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¥ 1,250.00

Cat# AP51857

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Anti-SLC6A8 Antibody

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