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Anti-XTRP2 Antibody

Western blot analysis of XTRP2 expression in Jurkat (A), THP1 (B), Raw264.7 (C), PC12 (D) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 71 kD)
Immunofluorescent analysis of XTRP2 staining in HCCLM3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q96N87
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	70897 Da

Additional Information
Gene ID	348932
Other Names	XTRP2; Sodium-dependent neutral amino acid transporter B(0)AT3; Sodium- and chloride-dependent transporter XTRP2; Solute carrier family 6 member 18; System B(0) neutral amino acid transporter AT3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human XTRP2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SLC6A18 (HGNC:26441)
Synonyms	B0AT3, XTRP2
Function	Does not show neutral amino acid transporter activity.
Cellular Location	Membrane; Multi-pass membrane protein
Tissue Location	Abundantly expressed in kidney, but not in intestine.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schmutz J.,et al.Nature 431:268-274(2004).
Kleta R.,et al.Nat. Genet. 36:999-1002(2004).
Eslami B.,et al.Tohoku J. Exp. Med. 208:25-31(2006).
Broer S.,et al.J. Clin. Invest. 118:3881-3892(2008).

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¥ 1,250.00

Cat# AP51858

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Anti-XTRP2 Antibody

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