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Anti-TCP1 zeta Antibody

Western blot analysis of TCP1 zeta expression in H1299 (A), mouse brain (B), PC12 (C) whole cell lysates. (Predicted band size: 53; 58 kD; Observed band size: 58 kD)
Immunofluorescent analysis of TCP1 zeta staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P40227
Reactivity	Human, Mouse, Rat, Rabbit, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	58024 Da

Additional Information
Gene ID	908
Other Names	CCT6; CCTZ; T-complex protein 1 subunit zeta; TCP-1-zeta; Acute morphine dependence-related protein 2; CCT-zeta-1; HTR3; Tcp20
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TCP1 zeta. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCT6A
Synonyms	CCT6, CCTZ
Function	Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed:25467444, PubMed:36493755, PubMed:35449234, PubMed:37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444).
Cellular Location	Cytoplasm.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Li W.-Z.,et al.J. Biol. Chem. 269:18616-18622(1994).
Lee Y.-K.,et al.Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Wang H.,et al.Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 424:157-164(2003).
Segel G.B.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:6060-6064(1992).

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Cat# AP51873

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Anti-TCP1 zeta Antibody

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