Anti-PBR Antibody

Western blot analysis of PBR expression in PMVEC (A), HepG2 (B), 3T3L1 (C), A2780 (D), A549 (E) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 18 kD)
Immunofluorescent analysis of PBR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P30536
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	18828 Da

Additional Information
Gene ID	706
Other Names	BZRP; MBR; Translocator protein; Mitochondrial benzodiazepine receptor; PKBS; Peripheral-type benzodiazepine receptor; PBR
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PBR. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TSPO
Synonyms	BZRP, MBR
Function	Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:24814875), but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral- type benzodiazepine receptor; can also bind isoquinoline carboxamides (PubMed:1847678).
Cellular Location	Mitochondrion membrane; Multi-pass membrane protein
Tissue Location	Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Riond J.,et al.Eur. J. Biochem. 195:305-311(1991).
Yakovlev A.G.,et al.Gene 155:201-205(1995).
Costa B.,et al.Mol. Pharmacol. 69:37-44(2006).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).

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Cat# AP51876

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Anti-PBR Antibody

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