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Anti-NICE4 Antibody

Western blot analysis of NICE4 expression in A549 (A), H1688 (B) whole cell lysates. (Predicted band size: 114 kD; Observed band size: 130 kD)
Immunofluorescent analysis of NICE4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q14157
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	114535 Da

Additional Information
Gene ID	9898
Other Names	KIAA0144; NICE4; Ubiquitin-associated protein 2-like; Protein NICE-4
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NICE4. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	UBAP2L (HGNC:29877)
Synonyms	KIAA0144, NICE4
Function	Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled nucleotide excision repair (TC-NER) machinery fails to resolve DNA damage (PubMed:35633597). Plays an important role in the activity of long-term repopulating hematopoietic stem cells (LT- HSCs) (By similarity). Is a regulator of stress granule assembly, required for their efficient formation (PubMed:29395067, PubMed:35977029). Required for proper brain development and neocortex lamination (By similarity).
Cellular Location	Nucleus. Chromosome. Cytoplasm Cytoplasm, Stress granule Note=Associates with nuclear chromatin.
Tissue Location	Ubiquitous..

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Nagase T.,et al.DNA Res. 2:167-174(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Marenholz I.,et al.Genome Res. 11:341-355(2001).
Brill L.M.,et al.Anal. Chem. 76:2763-2772(2004).

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¥ 1,250.00

Cat# AP51877

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Anti-NICE4 Antibody

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