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Anti-IL-22RA2 Antibody

Western blot analysis of IL-22RA2 expression in Hela (A) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 31 kD)
Immunofluorescent analysis of IL-22RA2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q969J5
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	30550 Da

Additional Information
Gene ID	116379
Other Names	Interleukin-22 receptor subunit alpha-2; IL-22 receptor subunit alpha-2; IL-22R-alpha-2; IL-22RA2; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; CRF2-10; Cytokine receptor family type 2, soluble 1; CRF2-S1; Interleukin-22-binding protein; IL-22BP; IL22BP; ZcytoR16
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-22RA2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	IL22RA2
Function	Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.
Cellular Location	Secreted.
Tissue Location	Expressed in placenta, spleen, breast, skin and lung. Also detected in intestinal tract, testis, brain, heart and thymus. No expression found in prostate, bladder, kidney, ovary, muscle, bone marrow, liver and uterus. Isoform 1 is expressed only in placenta. Isoform 2 is expressed in placenta and breast and at lower level in spleen, skin, thymus and stomach

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Gruenberg B.H.,et al.Genes Immun. 2:329-334(2001).
Kotenko S.V.,et al.J. Immunol. 166:7096-7103(2001).
Dumoutier L.,et al.J. Immunol. 166:7090-7095(2001).
Xu W.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:9511-9516(2001).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).

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¥ 1,250.00

Cat# AP51901

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Anti-IL-22RA2 Antibody

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