> 首页 > 产品 > 一抗 > 精选抗体 > G蛋白偶联受体抗体（GPCR） > Anti-Adenosine A2a Receptor Antibody

Anti-Adenosine A2a Receptor Antibody

Western blot analysis of Adenosine A2a Receptor expression in HEK293T (A), Hela (B), HepG2 (C), mouse brain (D), rat liver (E), mouse kidney (F) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 70; 45 kD)
Immunohistochemical analysis of Adenosine A2a Receptor staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Adenosine A2a Receptor staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P29274
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	44707 Da

Additional Information
Gene ID	135
Other Names	ADORA2; Adenosine receptor A2a
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Adenosine A2a Receptor. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ADORA2A
Synonyms	ADORA2
Function	Receptor for adenosine (By similarity). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (By similarity).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P30543}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P30543} Note=Colocalizes with GAS2L2 at neuronal processes {ECO:0000250\|UniProtKB:P30543}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Tiffany H.L.,et al.Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases.
Salvatore C.A.,et al.Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases.
Furlong T.J.,et al.Brain Res. Mol. Brain Res. 15:62-66(1992).
Le F.,et al.Biochem. Biophys. Res. Commun. 223:461-467(1996).
Puhl H.L. III,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,250.00

Cat# AP51941

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

reduce add

Availability:

询价

Anti-Adenosine A2a Receptor Antibody

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务