Anti-GPR176 Antibody

Western blot analysis of GPR176 expression in HEK293T (A), Hela (B), U2OS (C), mouse testis (D), mouse heart (E), rat testis (F), rat heart (G) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)
Immunofluorescent analysis of GPR176 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q14439
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	56998 Da

Additional Information
Gene ID	11245
Other Names	Probable G-protein coupled receptor 176; HB-954
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GPR176. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GPR176
Function	Orphan receptor involved in normal circadian rhythm behavior. Acts through the G protein subclass G(z)-alpha and has an agonist- independent basal activity to repress cAMP production.
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:Q80WT4}; Multi-pass membrane protein

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.