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Anti-GPR12 Antibody

Western blot analysis of GPR12 expression in HepG2 (A), Raw264.7 (B), rat heart (C) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 37 kD)
Immunofluorescent analysis of GPR12 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P47775
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	36730 Da

Additional Information
Gene ID	2835
Other Names	G-protein coupled receptor 12
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR12. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GPR12
Function	Brain-specific G protein-coupled receptor involved in regulating diverse physiological processes including neurite outgrowth, meiotic arrest, and lipid and carbohydrate metabolism (By similarity). Functions as a receptor with constitutive G(s)-mediated signaling activity that stimulates cyclic AMP (cAMP) production (PubMed:36593162). Also engages the G(i)/G(o) pathway, which counteracts the cAMP increase driven by G(s) activation (PubMed:12220620). Additionally, two lipids sphingosylphosphorylcholine (SPC) and sphingosine-1-phosphate (S1P) may act as endogenous ligand and activate GPR12 (PubMed:12220620).
Cellular Location	Cell membrane; Multi-pass membrane protein

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Song Z.-H.,et al.Genomics 28:347-349(1995).
Dunham A.,et al.Nature 428:522-528(2004).
Uhlenbrock K.,et al.Cell. Signal. 14:941-953(2002).
Yin H.,et al.J. Biol. Chem. 284:12328-12338(2009).

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¥ 1,250.00

Cat# AP51955

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Anti-GPR12 Antibody

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