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Anti-GPR50 Antibody

Western blot analysis of GPR50 expression in mouse testis (A), rat testis (B) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 67 kD)
Immunofluorescent analysis of GPR50 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q13585
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	67369 Da

Additional Information
Gene ID	9248
Other Names	Melatonin-related receptor; G protein-coupled receptor 50; H9
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR50. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GPR50
Function	G protein-coupled receptor that plays a role in numerous physiological processes including regulation of energy metabolism, neurite outgrowth or cell migration (PubMed:19699797). Promotes self- renewal and neuronal differentiation of neural progenitor cells through activation of the NOTCH and WNT/beta-catenin signaling pathways (By similarity). Modulates the KAT5-dependent glucocorticoid receptor signaling by modulating KAT5 subcellular compartmentalization (PubMed:21858214). Also plays a role in the activation TGFBR1 in the absence of TGFBR2 by interfering with FKBP1A binding to TGFBR1, leading to induction of both canonical and non-canonical SMAD signaling pathways resulting in inhibition of proliferation or promotion of migration (PubMed:29572483).
Cellular Location	Cell membrane; Multi-pass membrane protein. Postsynaptic density
Tissue Location	Hypothalamus and pituitary.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Reppert S.M.,et al.FEBS Lett. 386:219-224(1996).
Kaighin V.A.,et al.Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
Levoye A.,et al.EMBO J. 25:3012-3023(2006).
Bhattacharyya S.,et al.J. Lipid Res. 47:761-766(2006).

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¥ 1,250.00

Cat# AP51961

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50 µl

100 µl

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Anti-GPR50 Antibody

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