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Anti-Nav1.6 Antibody

Western blot analysis of Nav1.6 expression in MCF7 (A), Raw264.7 (B), H9C2 (C) whole cell lysates. (Predicted band size: 225 kD; Observed band size: 225 kD)
Immunofluorescent analysis of Nav1.6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9UQD0
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	225280 Da

Additional Information
Gene ID	6334
Other Names	MED; Sodium channel protein type 8 subunit alpha; Sodium channel protein type VIII subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.6
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Nav1.6. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SCN8A (HGNC:10596)
Synonyms	MED
Function	Pore-forming subunit of a voltage-gated sodium channel complex assuming opened or closed conformations in response to the voltage difference across membranes and through which sodium ions selectively pass along their electrochemical gradient (PubMed:24874546, PubMed:25239001, PubMed:25725044, PubMed:26900580, PubMed:29726066, PubMed:33245860, PubMed:36696443, PubMed:36823201). Contributes to neuronal excitability by regulating action potential threshold and propagation (PubMed:24874546, PubMed:25239001, PubMed:25725044, PubMed:26900580, PubMed:29726066, PubMed:33245860, PubMed:36696443, PubMed:36823201).
Cellular Location	Cell membrane; Multi-pass membrane protein. Cell projection, axon {ECO:0000250\|UniProtKB:Q9WTU3}. Note=Mainly localizes to the axon initial segment. {ECO:0000250\|UniProtKB:Q9WTU3}
Tissue Location	Expressed in the hippocampus with increased expression in epileptic tissue compared to normal adjacent tissue (at protein level) (PubMed:28842554).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Plummer N.W.,et al.J. Biol. Chem. 272:24008-24015(1997).
Plummer N.W.,et al.Genomics 54:287-296(1998).
Carrithers M.D.,et al.J. Biol. Chem. 284:8114-8126(2009).
Lin C.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases.
Jeong S.-Y.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP51970

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Anti-Nav1.6 Antibody

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