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Anti-Angiopoietin-4 Antibody

Western blot analysis of Angiopoietin-4 expression in A549 (A), H1792 (B) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)
Immunofluorescent analysis of Angiopoietin-4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9Y264
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	56849 Da

Additional Information
Gene ID	51378
Other Names	ANG3; ANG4; Angiopoietin-4; ANG-4; Angiopoietin-3; ANG-3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Angiopoietin-4. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ANGPT4
Synonyms	ANG3, ANG4
Function	Binds to TEK/TIE2, modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2. Promotes endothelial cell survival, migration and angiogenesis.
Cellular Location	Secreted.
Tissue Location	Highly expressed in the lung with much lower levels found in other tissues

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Nishimura M.,et al.FEBS Lett. 448:254-256(1999).
Valenzuela D.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:1904-1909(1999).
Deloukas P.,et al.Nature 414:865-871(2001).
Lee H.J.,et al.FASEB J. 18:1200-1208(2004).
Augustin H.G.,et al.Nat. Rev. Mol. Cell Biol. 10:165-177(2009).

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Cat# AP51972

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Anti-Angiopoietin-4 Antibody

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