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Anti-ECA39 Antibody

Western blot analysis of ECA39 expression in Jurkat (A), Ramos (B), NIHC3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
Immunofluorescent analysis of ECA39 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P54687
Reactivity	Human, Mouse, Rat, Rabbit, Pig, Bovine, Dog, Sheep, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42966 Da

Additional Information
Gene ID	586
Other Names	BCT1; ECA39; Branched-chain-amino-acid aminotransferase, cytosolic; BCAT(c); Protein ECA39
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ECA39. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BCAT1
Synonyms	BCT1, ECA39 {ECO:0000303\|PubMed:8692959}
Function	Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:P54690}.
Tissue Location	During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Schuldiner O.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:7143-7148(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Scherer S.E.,et al.Nature 440:346-351(2006).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).

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¥ 1,250.00

Cat# AP51975

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Anti-ECA39 Antibody

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