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Anti-Heme Oxygenase 2 Antibody

Western blot analysis of Heme Oxygenase 2 expression in HEK293T (A), Jurkat (B), A549 (C), mouse spleen (D), mouse lung (E), rat spleen (F), rat lung (G) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)
Immunofluorescent analysis of Heme Oxygenase 2 staining in PCCL cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P30519
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	36033 Da

Additional Information
Gene ID	3163
Other Names	HO2; Heme oxygenase 2; HO-2
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Heme Oxygenase 2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HMOX2
Synonyms	HO2
Function	[Heme oxygenase 2]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Cellular Location	Microsome membrane; Single-pass type IV membrane protein; Cytoplasmic side {ECO:0000250\|UniProtKB:P09601}. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P09601}; Single-pass type IV membrane protein; Cytoplasmic side {ECO:0000250\|UniProtKB:P09601}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Ishikawa K.,et al.J. Biol. Chem. 270:6345-6350(1995).
McCoubrey W.K. Jr.,et al.Arch. Biochem. Biophys. 295:13-20(1992).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Martin J.,et al.Nature 432:988-994(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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Cat# AP51976

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Anti-Heme Oxygenase 2 Antibody

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