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Anti-DROSHA Antibody

Western blot analysis of DROSHA expression in U87MG (A), PC3 (B), CT26 (C), C6 (D) whole cell lysates. (Predicted band size: 159 kD; Observed band size: 180 kD)
Immunofluorescent analysis of DROSHA staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9NRR4
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	159316 Da

Additional Information
Gene ID	29102
Other Names	RN3; RNASE3L; RNASEN; Ribonuclease 3; Protein Drosha; Ribonuclease III; RNase III; p241
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DROSHA. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	DROSHA
Synonyms	RN3, RNASE3L, RNASEN
Function	Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri- miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies. Plays a role in growth homeostasis in response to autophagy in motor neurons (By similarity).
Cellular Location	Nucleus. Nucleus, nucleolus. Cytoplasm {ECO:0000250\|UniProtKB:Q5HZJ0}. Note=A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
Tissue Location	Ubiquitous..

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Wu H.,et al.J. Biol. Chem. 275:36957-36965(2000).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Schmutz J.,et al.Nature 431:268-274(2004).
Gunther M.,et al.Mol. Cell. Biochem. 210:131-142(2000).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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¥ 1,250.00

Cat# AP52002

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100 µl

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Anti-DROSHA Antibody

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