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Anti-GSTM1 Antibody

Western blot analysis of GSTM1 expression in HeLa (A), HepG2 (B), rat testis (C) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 28 kD)
Immunohistochemical analysis of GSTM1 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P09488
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	25712 Da

Additional Information
Gene ID	2944
Other Names	GST1; Glutathione S-transferase Mu 1; GST HB subunit 4; GST class-mu 1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GSTM1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GSTM1 (HGNC:4632)
Synonyms	GST1
Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).
Cellular Location	Cytoplasm.
Tissue Location	Liver (at protein level).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Dejong J.L.,et al.Nucleic Acids Res. 16:8541-8554(1988).
Seidegaard J.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:7293-7297(1988).
Chen P.,et al.Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
Chen P.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP52008

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Anti-GSTM1 Antibody

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