> 首页 > 产品 > 一抗 > 其他 > Anti-HIC2 Antibody

Anti-HIC2 Antibody

Western blot analysis of HIC2 expression in HEK293T (A), BV2 (B) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 66; 90 kD)
Immunofluorescent analysis of HIC2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q96JB3
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	66156 Da

Additional Information
Gene ID	23119
Other Names	HRG22; KIAA1020; ZBTB30; Hypermethylated in cancer 2 protein; Hic-2; HIC1-related gene on chromosome 22 protein; Hic-3; Zinc finger and BTB domain-containing protein 30
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HIC2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HIC2
Synonyms	HRG22, KIAA1020, ZBTB30
Function	Transcriptional repressor.
Cellular Location	Nucleus.
Tissue Location	Highest levels in cerebellum.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Deltour S.,et al.Biochem. Biophys. Res. Commun. 287:427-434(2001).
Alliel P.M.,et al.Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
Kikuno R.,et al.DNA Res. 6:197-205(1999).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).

终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问，联系：0512-88856768 tech-china@abcepta.com.

我要评价

¥ 1,250.00

Cat# AP52010

Size:

50 µl

100 µl

Price:

¥ 1,250.00

¥ 2,050.00

Quantity:

reduce add

Availability:

询价

Anti-HIC2 Antibody

REFERENCES

相关产品

多肽合成服务

多克隆抗体制备服务

单克隆抗体制备服务

抗体测序服务

蛋白表达服务

药物发现定制服务